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Microbial Culturing Technique

Proper microbial culturing is essential for accurate laboratory results and maintaining pure cultures. This guide outlines the key steps in transferring bacteria to an agar slant medium, a common method used in microbiology labs.

1. Materials

• Agar slant medium
• Cultured plate with bacterial colonies
• Inoculation loop or needle
• Flame source (Bunsen burner)
• Cotton plugs

2. Procedure

1. Label the top of the agar slant tube with the name or number of the original culture, the date of inoculation, and the operator's group code.
2. Hold the bacterial plate in your left hand and the inoculation loop in your right. Sterilize the loop by passing it through the flame until it glows red, then allow it to cool slightly.
3. Using the cooled loop, gently pick up a well-isolated colony from the plate. Return the plate to its place, and hold the slant tube diagonally with your right hand. Use your little finger and palm to hold the cotton plug while carefully removing it. Pass the tube mouth over the flame to sterilize it.
4. Insert the loop into the agar at the bottom of the slant tube. Draw a straight line from the bottom along the surface of the slant, then create a zigzag pattern. Alternatively, you can draw a curved line without a straight path. After removing the loop, sterilize the tube mouth again with flame, replace the cotton plug, and set the tube aside. Sterilize the loop by flaming it before setting it down.
5. If the bacteria were originally grown in a liquid culture, hold the slant tube and the original strain tube together with your left hand. With your right hand, use your ring and little fingers to hold both cotton plugs. Flame the tube mouth and the loop, take the culture from the original tube, and immediately transfer it to the slant surface. Replace the cotton plug, flame the loop, and place everything back on the rack.
6. Incubate the slant tube in a 37°C incubator for 24 hours. After incubation, a uniform lawn should appear on the surface of the slant, indicating successful growth.

Qingdao Weisi Biotechnology Co., Ltd. is a leading company in the biotechnology industry, providing high-quality products such as microbial culture media, reference substances, standard strains, ELISA kits, test strips, serum, blank plates, and other lab consumables. The company serves a wide range of sectors including food, pharmaceuticals, cosmetics, research institutions, schools, hospitals, and water testing laboratories. Committed to quality and customer satisfaction, Qingdao Weisi ensures that every client receives reliable service and premium products tailored to their needs.

Whether you're a seasoned scientist or just starting out in the lab, Qingdao Weisi is here to support your research and testing requirements with professionalism and care.

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