Mouse acidic fibroblast growth factor 1 ELISA kit detection method - Database & Sql Blog Articles

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Mouse Acidic Fibroblast Growth Factor 1 (FGF1) ELISA Kit: Detection Method

Fibroblast Growth Factor (FGF) exists in two closely related forms: Basic Fibroblast Growth Factor (bFGF) and Acidic Fibroblast Growth Factor (aFGF). Both share a common cell surface receptor and exhibit broad biological activity, often regulated by TGF-β. The Mouse Acidic Fibroblast Growth Factor 1 ELISA Kit is designed for precise detection of FGF1 levels in various biological samples. This kit is part of a comprehensive range that includes kits for Chicken bFGF, Human bFGF-9, and more. For detailed procedures, please contact us directly.

The following are standard methods used in routine analysis:

1. A 12-well cell culture plate was prepared with 0.5 ml of cell culture medium containing 1 x 10⁴ bovine adrenal vascular endothelial cells (ACE) per well.
2. The sample to be tested was diluted in PBS containing 0.2% gelatin. Then, 0.5 ml of the diluted sample was added to each well. The same concentration of sample was replaced every other day. A control well received 0.5 ml of cell culture solution. The plates were incubated at 37°C in a 5% CO₂ atmosphere with saturated humidity for 4–5 days.
3. Cell growth was quantified using the MTT assay, [³H] deoxythymidine incorporation method, or direct cell counting after trypsin digestion. Dose-response curves were plotted, and the highest dilution that resulted in a 50% maximal proliferative response was defined as 1 U of FGF.

Product Detailed Information

Chinese Name: Mouse Acidic Fibroblast Growth Factor (FGF1) Test Kit

English Name: ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1)

Product Specifications: 48T / 96T

Product Price: Inquiry

Detection Range: Refer to the ELISA Kit Instructions for full details.

Data Processing and Results Reporting

1. Calculate recovery rate: Recovery Rate 1 = ...
2. Calculate the average recovery rate:
3. Calculate the proportional system error: Proportional System Error = 100% - Average Recovery Rate
4. Acceptable Judgment: The proportional system error must not exceed 1/2 of the total error allowed by CLIA 88.

This ELISA Kit provides a reliable and accurate method for measuring FGF1 levels, making it an essential tool for researchers and laboratories involved in cellular signaling and growth factor studies. Whether you're working on basic research or clinical applications, this kit ensures consistent and reproducible results.