Human adrenomedullin (ADM) enzyme-linked kit price - Database & Sql Blog Articles

**Human Adrenomedullin (ADM) Enzyme-Linked Immunoassay Kit – Instructions for Use** This kit is intended for research use only and is designed to quantify human adrenomedullin (ADM) in serum, plasma, and other biological fluids. The detection range of the kit is from 2 ng/L to 48 ng/L. **Principle of the Assay** The Human ADM ELISA Kit employs a double-antibody sandwich method. A microtiter plate is pre-coated with purified anti-ADM antibodies. After incubation with the sample, the target ADM binds to the immobilized antibody. An HRP-conjugated secondary antibody is then added, forming a complex. Following washing steps, the substrate TMB is introduced, which turns blue under HRP catalysis and then yellow when an acid stop solution is added. The intensity of the color is directly proportional to the ADM concentration in the sample. Absorbance is measured at 450 nm using a microplate reader, and the ADM concentration is determined by comparing the sample’s OD value to a standard curve. **Kit Components** - 130× Washing Solution: 20 mL × 1 bottle - Stop Solution: 6 mL × 1 bottle - Enzyme Standard Reagent: 6 mL × 1 bottle - Standard Product (96 ng/L): 0.5 mL × 1 bottle - Enzyme Labelled Plate: 12 wells × 8 - Standard Dilutions: 1.5 mL × 1 bottle - Sample Diluent: 6 mL × 1 bottle - Instruction Manual: 1 part - Color Reagent A: 6 mL × 1 bottle - Sealing Film: 2 sheets - Color Reagent B: 6 mL × 1 bottle - Sealed Bag: 1 piece **Sample Requirements** Samples should be processed as soon as possible after collection. If not tested immediately, store at -20°C, avoiding repeated freeze-thaw cycles. Avoid using samples containing NaN3, as it may inhibit HRP activity. **Procedure Summary** 1. **Standard Dilution**: Prepare serial dilutions of the original standard according to the provided table. 2. **Sample Addition**: Add 50 μL of standard, 40 μL of sample diluent, and 10 μL of sample to the appropriate wells. 3. **Incubation**: Seal the plate and incubate at 37°C for 30 minutes. 4. **Washing**: Wash the plate five times with diluted washing buffer. 5. **Enzyme Addition**: Add 50 μL of enzyme-labeled reagent to each well except the blank. 6. **Second Incubation**: Repeat incubation at 37°C for 30 minutes. 7. **Color Development**: Add 50 μL of Color Reagent A and B, mix gently, and incubate at 37°C for 15 minutes. 8. **Stop Reaction**: Add 50 μL of stop solution to terminate the reaction. 9. **Measurement**: Read absorbance at 450 nm within 15 minutes of adding the stop solution. **Data Analysis** Plot the standard curve using the OD values against the known concentrations. Calculate the sample concentration based on the regression equation or by interpolating from the standard curve. Multiply by the dilution factor if applicable. **Notes** - Allow the kit to reach room temperature before use. Store unopened enzyme reagents in a sealed bag. - If the washing solution crystallizes, warm it in a water bath before use. - Use dedicated pipettes and ensure accuracy. Control loading time to avoid errors. - Always prepare a standard curve and consider diluting high-concentration samples. - Use the sealing film only once to prevent contamination. - Keep the substrate away from light. - Follow the manual strictly; results must be confirmed with a microplate reader. - All samples and waste should be treated as biohazardous materials. - Do not mix components from different batches. - In case of conflict, the English version of the manual takes precedence.

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