EL-C1600N100013-B
Why do dual wavelengths need to be used when reading a microplate reader? This question often comes up among customers during ELISA kit testing. To fully understand the importance, it's essential to follow the correct procedure. In this section, Shanghai Jinma Bio explains why using dual wavelengths is necessary for accurate readings with the microplate reader. Let’s dive in and learn more!
First, before using the microplate reader, it's important to preheat it for 10–15 minutes. This helps stabilize the instrument and ensures more reliable results. When performing an ELISA test, using two wavelengths to measure absorbance is key. This technique helps eliminate interference that may occur during single-wavelength detection—such as background color, sample concentration, or other contaminants. Typically, the maximum wavelength is chosen as the reference. At this reference wavelength, the absorbance of the target substance is minimal, allowing the difference between the detection and reference wavelengths to account for non-specific absorption. As a result, dual-wavelength measurements significantly reduce the impact of fingerprints, impurities, and other optical interferences, ensuring greater accuracy in your experimental data.
At Shanghai Jinma Bio, we carefully select high-quality imported antibodies, standards, chromogenic reagents, and ELISA plates to ensure optimal performance. Our products incorporate advanced patented technology and are manufactured following strict quality control standards. We also use batch-imported raw materials and our own specially formulated diluents to maintain high performance while keeping costs manageable. If you're looking for reliable ELISA kits, feel free to contact us today. We're here to help you achieve accurate and consistent results in your experiments.
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