EL-C1600N100013-B
Why do we need to use dual wavelengths when reading with a microplate reader? This is a common question from customers during ELISA kit testing. To fully understand, it's important to follow the proper procedures. In this section, Shanghai Jinma Bio explains why dual wavelength readings are essential for accurate results. Let’s dive in and learn more!
First, before using the microplate reader, it should be preheated for 10–15 minutes to ensure stable performance and reliable data. Next, ELISA tests typically use two wavelengths to measure absorbance. This method helps eliminate interference that might occur with single-wavelength detection, such as background color or sample concentration issues. Usually, the maximum wavelength is chosen as the reference wavelength. At this point, the absorbance of the target substance is minimal. The difference between the detection wavelength and the reference wavelength can effectively reduce non-specific absorption. As a result, dual-wavelength measurement minimizes the impact of fingerprints, impurities, and other contaminants, ensuring the accuracy of your experimental results.
At Shanghai Jinma Bio, we carefully select high-quality imported antibodies, standards, chromogenic reagents, and ELISA plates to guarantee optimal performance. Our products incorporate advanced patented technology and are manufactured following strict quality control protocols. We also import raw materials in batches and use our own special formulation solutions to maintain high performance while keeping costs reasonable. If you're looking for a reliable ELISA kit, feel free to contact us today. We’re here to help!
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