Human adrenomedullin (ADM) enzyme-linked kit price - Database & Sql Blog Articles

**Human Adrenomedullin (ADM) Enzyme-Linked Immunoassay Kit – Instructions for Use** This kit is intended for research use only and is designed to quantify human adrenomedullin (ADM) in serum, plasma, and other biological fluids. The detection range is from 2 ng/L to 48 ng/L. **Principle of the Assay** The Human ADM ELISA Kit employs a sandwich immunoassay technique. A microtiter plate is pre-coated with a specific antibody against ADM. After incubation with the sample, the target antigen binds to the immobilized antibody. An HRP-conjugated secondary antibody then recognizes the captured ADM, forming an antibody-antigen-enzyme complex. Following washing steps, the TMB substrate is added, which changes color in the presence of HRP. The reaction is stopped with a stop solution, and the resulting color intensity is measured at 450 nm using a microplate reader. The absorbance value is directly proportional to the ADM concentration in the sample. **Kit Components** - 130x Washing Solution: 20 ml × 1 bottle - Stop Solution: 6 ml × 1 bottle - Enzyme Standard Reagent: 6 ml × 1 bottle - Standard (96 ng/L): 0.5 ml × 1 bottle - Enzyme-Labeled Plate: 12 wells × 8 strips - Standard Diluent: 1.5 ml × 1 bottle - Sample Diluent: 6 ml × 1 bottle - Instruction Manual: 1 copy - Color Reagent A: 6 ml × 1 bottle - Color Reagent B: 6 ml × 1 bottle - Sealing Film: 2 sheets - Sealed Bag: 1 piece **Sample Requirements** Samples should be processed as soon as possible after collection. If not tested immediately, store at -20°C and avoid repeated freeze-thaw cycles. Samples containing NaN3 should not be used, as it may inhibit HRP activity. **Procedure Overview** 1. **Standard Dilution**: Prepare serial dilutions of the standard according to the provided chart. 2. **Add Sample**: Load 50 µL of standard and 40 µL of sample diluent into designated wells, followed by 10 µL of the sample (final 5x dilution). 3. **Incubation**: Seal the plate and incubate at 37°C for 30 minutes. 4. **Washing**: Wash the plate 5 times with diluted washing buffer. 5. **Add Enzyme Conjugate**: Add 50 µL of enzyme-labeled reagent to each well except the blank. 6. **Incubation**: Incubate again at 37°C for 30 minutes. 7. **Color Development**: Add 50 µL of Color Reagent A and B sequentially, incubate at 37°C for 15 minutes. 8. **Stop Reaction**: Add 50 µL of stop solution to each well. 9. **Measurement**: Read OD values at 450 nm within 15 minutes of stopping the reaction. **Data Analysis** Plot the standard curve using the OD values and corresponding concentrations. Calculate the sample concentration using linear regression or direct interpolation. Multiply by the dilution factor to obtain the actual concentration. **Notes** - Allow the kit to reach room temperature before use. Store unopened plates in a sealed bag. - Concentrated wash solution may crystallize; warm it gently if needed. - Use dedicated pipettes and ensure accuracy. - Always run a standard curve and consider sample dilution if OD exceeds the standard range. - Do not reuse sealing films to prevent cross-contamination. - Keep substrates away from light. - Follow the manual strictly. - All samples and waste must be handled as biohazardous materials. - Do not mix components from different batches. - In case of discrepancy, the English manual takes precedence.

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